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A , B CCK-8 assay to detect the inhibitory effect of NRG1 on cell death induced by Erastin, <t>Nigericin,</t> Hydroxyurea, and Wogonoside in BC cells ( n = 3). C Transmission electron microscopy to observe mitochondrial damage in MDA-MB-231 cells. Scale bar = 2 μm (Zoom in = 1 μm). D , E Western blot to detect the effect of exogenous NRG1 on the AKT/mTOR pathway in BC cells ( n = 3). F , G Western blot to observe the effect of CAF-derived NRG1 on the AKT/mTOR pathway in BC cells ( n = 3). Data are expressed as the mean ± S.D. for all panels: ns = no significance, # P < 0.05, ** , ## P < 0.01, ***,### P < 0.001.
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A , B CCK-8 assay to detect the inhibitory effect of NRG1 on cell death induced by Erastin, <t>Nigericin,</t> Hydroxyurea, and Wogonoside in BC cells ( n = 3). C Transmission electron microscopy to observe mitochondrial damage in MDA-MB-231 cells. Scale bar = 2 μm (Zoom in = 1 μm). D , E Western blot to detect the effect of exogenous NRG1 on the AKT/mTOR pathway in BC cells ( n = 3). F , G Western blot to observe the effect of CAF-derived NRG1 on the AKT/mTOR pathway in BC cells ( n = 3). Data are expressed as the mean ± S.D. for all panels: ns = no significance, # P < 0.05, ** , ## P < 0.01, ***,### P < 0.001.
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A , B CCK-8 assay to detect the inhibitory effect of NRG1 on cell death induced by Erastin, Nigericin, Hydroxyurea, and Wogonoside in BC cells ( n = 3). C Transmission electron microscopy to observe mitochondrial damage in MDA-MB-231 cells. Scale bar = 2 μm (Zoom in = 1 μm). D , E Western blot to detect the effect of exogenous NRG1 on the AKT/mTOR pathway in BC cells ( n = 3). F , G Western blot to observe the effect of CAF-derived NRG1 on the AKT/mTOR pathway in BC cells ( n = 3). Data are expressed as the mean ± S.D. for all panels: ns = no significance, # P < 0.05, ** , ## P < 0.01, ***,### P < 0.001.

Journal: Cell Death Discovery

Article Title: NRG1/PDGFC loop between fibroblasts and cancer cells drives paclitaxel resistance via ferroptosis suppression in breast cancer

doi: 10.1038/s41420-025-02785-2

Figure Lengend Snippet: A , B CCK-8 assay to detect the inhibitory effect of NRG1 on cell death induced by Erastin, Nigericin, Hydroxyurea, and Wogonoside in BC cells ( n = 3). C Transmission electron microscopy to observe mitochondrial damage in MDA-MB-231 cells. Scale bar = 2 μm (Zoom in = 1 μm). D , E Western blot to detect the effect of exogenous NRG1 on the AKT/mTOR pathway in BC cells ( n = 3). F , G Western blot to observe the effect of CAF-derived NRG1 on the AKT/mTOR pathway in BC cells ( n = 3). Data are expressed as the mean ± S.D. for all panels: ns = no significance, # P < 0.05, ** , ## P < 0.01, ***,### P < 0.001.

Article Snippet: PDGFC recombinant protein (HY-P7279), Paclitaxel (PTX, HY-B0015), ARQ 751 trihydrochloride (HY-137458A), Erastin (HY-15763), Nigericin (HY-127019), Hydroxyurea (HY-B0313), Wogonoside (HY-N0399), and PDGFRα kinase inhibitor 1 (HY-111507) were purchased from MedChemExpress (USA), while NRG1 recombinant protein was obtained from Cell Signaling Technology (26941).

Techniques: CCK-8 Assay, Transmission Assay, Electron Microscopy, Western Blot, Derivative Assay